After 13 hours, the Eastbound 401 has reopened at Queens Line in Tilbury.
Provincial Police have removed barricades closing the Eastbound 401 at Queen's Line.
A 30-year-old male truck driver from Brampton has been charged with careless driving from a rollover crash that shut down the highway in Tilbury late last night.
The OPP says around 10:30pm an eastbound tractor trailer, travelling through the construction zone near Dillon Rd., rolled onto its side and ended up in the ditch.
The river was transported to hospital with non-life threatening injuries.